Journal of Korean Medicine Rehabilitation : eISSN 2288-4114 / pISSN 1229-1854

Fig. 3.

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Fig. 3. Effect of HWJ on osteogenesis of MC3T3-E1 cells. We used ALP staining to analyze whether HWJ promotes differentiation of pre-osteoblast (MTCT3-E1 cell). MC3T3-E1 cells were stimulated by osteoinductive media in presence or absence of indicated HWJ concentrations for 7 days and subjected to ALP staining. The stained cells were visualized using Leica DM IL LED microscope at 500× and 200× magnification. This picture shows that the color of ALP staining below the concentration of HWJ 62.5 μg/mL are darker than that of other groups. HWJ: Hwaweo-Jeon, ALP: alkaline phosphatase.
J Korean Med Rehabi 2020;30:13-29
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