Fig. 10. Effect of DG on osteocalcin and Runx2 expression in MG63 cells. The MG63 cells were cultivated with 1,25-(OH)₂D3 (calcitriol) or DG (500 μg/mL) for 24 hours. The total RNAs were isolated from the cells and used for semi-quantitative RT-PCR with (A) osteocalcin or (B) Runx2 specific primers. The PCR products were analyzed with gel electrophoresis. Nor: vehicle-treated group, Cal: calcitriol-treated group, DG: Danggwisu-san treated group. Runx2: runt-related transcription factor 2, RT-PCR: real-time polymerase chain reaction.

J Korean Med Rehabi 2021;31:1-16 https://doi.org/10.18325/jkmr.2021.31.1.1

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