Journal of Korean Medicine Rehabilitation : eISSN 2288-4114 / pISSN 1229-1854

Fig. 2.

Download original image
Fig. 2. Effect of NTB on NO production and iNOS expression in LPS-stimulated RAW264.7 cells. (A) RAW264.7 cells were treated with NTB for 2 hours and then stimulated with LPS (500 ng/mL) for 24 hours. The Griess reagent was used to measure the amount of NO secreted in the cell culture supernatant. (B) Effect of NTB on the expression of iNOS in LPS-stimulated RAW264.7 cells. RAW264.7 cells were pretreated with different concentrations of NTB or Bay11-7082 (20 μM) for 2 hours and then treated with LPS (200 ng/mL) for 24 hours. The protein expression of iNOS was confirmed by Western blot analysis. Bay11-7082 was used as a positive control. A house keeping protein β-actin was used as a control protein. The protein bands of iNOS were quantitatively evaluated by C300. Experimental data were presented as means±standard error of the mean (++p<0.01 compared with the control group, +++p<0.001 compared with the control group and *p<0.05, **p<0.01, ***p<0.001 compared with LPS groups). NTB: Naetakbaekryeom-san, NO: nitric oxide, iNOS: inducible NO synthase, LPS: lipopolysaccharides.
J Korean Med Rehabi 2022;32:9-18 https://doi.org/10.18325/jkmr.2022.32.4.9
© 2022 J Korean Med Rehabi