Journal of Korean Medicine Rehabilitation : eISSN 2288-4114 / pISSN 1229-1854

Fig. 4.

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Fig. 4. Effect of NTB on NF-κB activation in PMA-stimulated cells. Both (A) RAW264.7 cells and (B) 293T cells seeded into poly-D-lysine hydrobromide-coated 96-well plates were transfected by pNF-κB-SEAP DNA using Hily Max. And then the cells were treated NTB with different concentrations or Bay11-7082 (20 µM) for 2 hours and stimulated with PMA (30 ng/mL) for 24 hours. SEAP activity was determined using the QUANTI-Blue assay system. Experimental data were presented as means±SEM from three independent experiments. ±SEM from three independent experiments. *p<0.05, **p<0.01. NTB: Naetakbaekryeom-san, NF-κB: nuclear factor kappa-light-chain-enhancer of activated B cells, PMA: phorbol 12-myristate 13-acetate, SEM: standard error of the mean. +P<0.05 compared with the control group, ++P<0.01 compared with the control group.
J Korean Med Rehabi 2022;32:9-18 https://doi.org/10.18325/jkmr.2022.32.4.9
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