Fig. 1. Inhibitory effects of EEPF on D-gal-induced reduction of cell viability and PCNA expression in C2C12 cells. Cells were treated with various concentrations of D-gal for 24 h. (A) or treated with EEPF for 1 h, and then stimulated with D-gal for 24 h (B-D). (A-C) The results of quantitative analysis of cell viability according to the CCK-8 assay were presented. The data were represented as mean±SD of three independent experiments. (D) After collecting cells and isolating total protein, changes in PCNA expression were detected using Western blot analysis. β-actin was used as a loading control. EEPF: ethanol extract of Perilla frutescens leaf, PNCA: proliferating cell nuclear antigen, CCK-8: cell counting kit 8, SD: standard deviation. Significant differences compared with the control cells (*p<0.05 and ^†p<0.001) or D-gal-treated cells (^‡p<0.05 and ^§p<0.001) were shown.

J Korean Med Rehabi 2024;34:15-28 https://doi.org/10.18325/jkmr.2024.34.2.15

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