Fig. 2. Inhibition of D-gal-induced apoptosis by EEPF in C2C12 cells. (A and B) Cells were fixed, stained with annexin V/PI, and then analyzed by flow cytometry. (A) The percentages of apoptotic cells were determined by counting the percentage of annexin V+ cells. (B) The results of flow cytometry analysis were expressed as the mean±SD. (C and D) After staining with DAPI, the morphology of the nucleus was observed. Representative photomicrographs of nuclei after DAPI staining (C) and quantified results (D) were shown. EEPF: ethanol extract of Perilla frutescens leaf, Annexin V/PI: Annexin V-Propidium iodide, DAPI: 4′,6-diamidino-2-phenylindol. Significant differences compared with the control cells (*p<0.001) or D-gal-treated cells (^†p<0.001) were shown.

J Korean Med Rehabi 2024;34:15-28 https://doi.org/10.18325/jkmr.2024.34.2.15

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