Journal of Korean Medicine Rehabilitation : eISSN 2288-4114 / pISSN 1229-1854

Fig. 4.

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Fig. 4. Protection of D-gal-induced ROS generation and mitochondrial dysfunction by EEPF in C2C12 cells. (A) or 24 h (B) with or without D-gal (200 mM). After collecting cells and staining with (A) DCF-DA or (B) JC-1, the levels of ROS generation and JC-1 monomers were determined using a flow cytometer. Percentages of (A) DCF-positive cells and (B) cells with JC-1 monomers were displayed as bars and the results were expressed as the mean±SD. ROS: reactive oxygen species, EEPF: ethanol extract of Perilla frutescens leaf, DCF-DA: 2',7'-dichlorofluorescein diacetate, JC-1: 5,5',6,6'-tetrachloro-1,1'3,3'-tetraethyl-imidacarbocyanune iodide. *p<0.001 compared with the control group, p<0.001 compared with the D-gal-treated group.
J Korean Med Rehabi 2024;34:15-28 https://doi.org/10.18325/jkmr.2024.34.2.15
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