Fig. 5. Attenuation of D-gal-induced cellular senescence and DNA damage by EEPF in C2C12 cells. (A and B) SA-β-gal staining was performed using the SA-β-gal kit according to the manufacturer's protocol to confirm cellular senescence. Representative cell images (A) of SA-β-gal staining and their activity (B) were presented. (C) After treatment, cells were double-stained with γH2AX (red) and DAPI (blue), and representative immunofluorescence images observed were shown. EEPF: ethanol extract of Perilla frutescens leaf, SA-β-gal: senescence-associated beta-galactosidase, DAPI: 4′,6-diamidino-2-phenylindol. *p<0.001 compared with the control group, ^†p<0.001 compared with the D-gal-treated group.

J Korean Med Rehabi 2024;34:15-28 https://doi.org/10.18325/jkmr.2024.34.2.15

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