Fig. 6. Suppression of D-gal-induced senescence markers and inflammatory response by EEPF in C2C12 cells. (A) The levels of the indicated proteins were detected using western blot analysis. The production amounts of (B) IL-6 and (C) IL-18 using the cell culture supernatants were detected using corresponding cytokine detection kits according to their manufacturer's instructions. All data were expressed as the mean±SD. EEPF: ethanol extract of Perilla frutescens leaf, IL: Interleukin. *p<0.001 compared with the control group, †p<0.001 compared with the D-gal-treated group.
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