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Effects of Jinmu-tang on the Osteoarthritis by MIA in Rats
J Korean Med Rehabil 2018;28:19-31
Published online January 31, 2018;  https://doi.org/10.18325/jkmr.2018.28.1.19
Copyright © 2018 The Society of Korean Medicine Rehabilitation.

Doo-Hwa Yang, K.M.D., Chang-Hoon Woo, K.M.D., Hee-Duk An, K.M.D.

Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University
Correspondence to: Hee-Duk An, Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Hanny University, 136 Sincheondong-ro, Suseong-gu, Daegu 42158, Korea
TEL (053) 770-2109
FAX (053) 770-2055
E-mail okee@dhu.ac.kr
Received December 29, 2017; Revised January 16, 2018; Accepted January 17, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objectives The object of this study was to investigate the antioxidative and antiinflammatory effects of Jinmu-tang extract (JMT) on the Monosodium iodoacetate (MIA)-induced rat osteoarthritis.
Methods To investigate the antioxidant capacities of JMT, we measured the total polyphenol and flavonoid, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-Azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. To evaluate the antioxidative and antiinflammatory effects of JMT, the rats were divided into 5 groups (n=8). Normal group was not induced by MIA and treated at all (N), control group was induced by MIA and not treated at all (Con), positive control group was induced by MIA and orally administered indomethacin 5 mg/kg (Indo) and experimental groups were induced by MIA and orally administered JMT 100 mg/kg (JMT100) and JMT 200 mg/kg (JMT200) for 4 weeks. The changes of anti-type II collagen antibody in serum, heme oxygenase-1 (HO-1), phosphorylated inhibitor of κBα (p-IκBα), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNF-α) in knee joint tissue and histopathological observation (Hematoxylin & Eosin and Safranin-O stain) were measured.
Results Total polyphenol and flavonoid levels of JMT were 26.90±0.33 mg/g and 6.02±0.34 mg/g. IC50 of L-ascorbic acid and JMT of DPPH radical scavenging activity were 1.35±0.07 μg/ml and 52.95±0.97 μg/ml. IC50 of L-ascorbic acid and JMT of ABTS radical scavenging activity were 3.18±0.02 μg/ml and 91.49±1.74 μg/ml. In serum, the anti-type II collagen antibody levels of JMT100 and JMT200 groups were decreased significantly. In knee joint tissue, the HO-1 level of JMT200 was increased significantly. The p-IκBα and TNF-α levels of JMT200 were decreased significantly. The COX-2 and iNOS levels of JMT groups were decreased significantly. In histopathological observation, in comparison with Con, synovial tissue, cartilage and proteoglycan of JMT100 and JMT200 were well preserved.
Conclusions According to the results, It is considered that JMT has antioxidant and antiinflammatory effects for MIA-induced rat osteoarthritis, so it could be applied to osteoarthritis treatment.
Keywords : Jinmu-tang, Osteoarthritis, Monosodium iodoacetate (MIA), Antiinflammation, Antioxidation


January 2018, 28 (1)